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Hepatic cancer pathophysiology, Mult mai mult decât documente.

HBV infection of a wide variety of cell types has been reported, but productive infection and pathology appear to be limited to the liver.

Among the many cell types found in the liver, HBV infects the hepatocyte, the major parenchymal cell. Following infection, virus is shed from hepatocytes into the bloodstream, so that every hepatocyte may become infected.

CHRONIC HEPATITIS B VIRUS INFECTIONS - The Infectious Etiology of Chronic Diseases - NCBI Bookshelf

During the peak of an infection, titers of virus in the blood may reach per cubic centimeter. Infection of hepatocytes is not typically cytopathic, and the liver pathology results from the immune response to the infected cells. Depending on the strength of the immune response, infections may be either transient or chronic.

Transient infections generally resolve in fewer than 6 months, while chronic infections may be lifelong. When a hepatocyte is infected, the viral DNA genome is transported to hepatic cancer pathophysiology nucleus, where it is converted from a relaxed circular DNA to a covalently closed circular form cccDNAwhich serves as the template for viral mRNA synthesis.

  1. Tema plagiatului este tot mai mult discutată în ultima vreme.
  2. Comentarii This book covers all liver tumors and lesions that clinically and radiologically mimic liver tumors.

Though the coding capacity of HBV is limited, it is still capable of encoding three envelope proteins, a nucleocapsid protein, a transcriptional transactivator, and a reverse transcriptase RT.

Encoding of the reverse transcriptase, the largest HBV protein, requires almost the entire viral genome. To facilitate this, the reverse transcriptase is encoded in different translational reading frames than the other viral gene products, so that overlapping reading hpv virus not 16 or 18 can be utilized.

Following completion of reverse transcription, the RT then synthesizes hepatic cancer pathophysiology, but not all of the second DNA strand, to recreate the partially double stranded virion DNA. Prior to completion of the second strand, nucleocapsids are packaged into viral envelopes by budding into the endoplasmic reticulum, and virions are exported from the cell.

Early after infection, and probably after division of an infected hepatocyte, extra cccDNA is synthesized, maintaining the copy number at 5 to 50 per hepatic cancer pathophysiology.

Transmission Transmission is parenteral, requiring exposure to the blood or blood-contaminated materials of infected individuals.

Molecular Genetics of Liver Neoplasia

The most common mode of exposure leading to chronic infection occurs at birth when the mother is chronically infected, or during the first year of life. During this period, the risk of an infection becoming chronic is at least 90 percent. In contrast, the risk of chronic infection in adults is greater than 10 percent. According to the CDC, the hepatic cancer pathophysiology common exposure risks in adults in the United States are sexual activity 50 percent of cases and intravenous drug abuse 15 percent of cases.

Liver Pathophysiology and Schematic Diagram

Public Health Issues Prevalence The case fatality rate in adults due to acute hepatitis is about 1 percent. According to WHO, there are now million chronically infected individuals worldwide. Of these, 60 million are expected to die prematurely liver cancer or cirrhosis, at a rate of approximately 1 million per year 5, per year hepatic cancer pathophysiology the United States.

This does not account for new cases, which will continue to accumulate in the coming decades. Vaccines A vaccine comprised of the viral envelope proteins has been available for over 20 years.

Tumors and Tumor-Like Lesions of the Hepatobiliary Tract

Due in part to high cost, universal vaccination was not initially feasible in many parts of the world, but lower hepatic cancer pathophysiology vaccines have subsequently come into use. Universal vaccination of school children is now in effect in the United States.

In some parts of the world, especially in Africa and regions of Asia, chronic infection rates exceed 5—10 percent of the population, but vaccination has not yet been economically feasible in all of these areas, even hepatic cancer pathophysiology low-cost vaccines. Although attempts are under way to address this problem Kane,for various reasons of cost and delivery, HBV is likely to remain a major public health problem.

On top of this problem there is evidence for vaccine escape mutants He et al. Though these do not yet seem to be a major public health problem, they remain a concern even for the large pool of individuals that have already received the current vaccine. In addition, about 5 percent of vaccinated individuals fail to produce a measurable antibody response, suggesting that they also remain at risk for HBV infection. Current Research A major goal of current research has thus been the development of therapies to cure chronically infected individuals.

A hepatic cancer pathophysiology in achieving this is that hepatocytes comprise a self-renewing population with a low turnover rate, and this population often appears to be percent infected.

This same barrier is confronted and overcome during immune clearance of transient infections, though it remains controversial how the virus is actually destroyed Guidotti et al. However, in chronic carriers, the immune system is usually unable to mount such a response, especially in those infected as children.

Some hope for better immunotherapies has however been sustained by papillary thyroid cancer emedicine fact that interferon alpha administration induces virus loss in about 20—30 percent of carriers Hoofnagle and Lau,typically those with adult-acquired infections.

In addition, some carriers experience spontaneous loss of the virus in association with a flare of liver disease. In both instances, clearance is probably due to activation of the same hpv cancer male symptoms of hepatic cancer pathophysiology responses that are active in clearance of transient infections. Key issues now are how this clearance is carried out, whether it requires destruction of all of the infected hepatocytes, if the immune system has the capacity to cure an infected hepatocyte, and if it can be induced in carriers that have failed to respond to interferon therapy with virus clearance.

Treatment Another approach to treatment of chronic infections is administration of nucleoside analog inhibitors of the HBV reverse transcriptase. Lamivudine was approved by the U. Food and Drug Administration FDA in and has been shown in clinical trials to have a treatment success rate similar to interferon alpha Perrillo, A significant problem with lamivudine is the emergence of drug-resistant hepatic cancer pathophysiology of HBV as therapy continues past a virus del papiloma humano ano en mujeres. Another nucleoside, adefovir dipivoxil, recently received FDA approval and to date drug-resistant variants have not been reported.

Moreover, this drug retains hepatic cancer pathophysiology against lamivudine-resistant HBV Delaney et al. However, at doses higher than used for HBV carriers, nephrotoxicity has been observed Tanji et al.

It may be that nephrotoxicity will become a problem in HBV therapy due to a cumulative effect if carriers require treatment indefinitely.

A number of other nucleoside analogs are now in Phase II trials. If these compounds are not toxic during long-term administration, and if viral multi-drug resistance does not develop, it should be possible to eliminate over time the viral cccDNA that maintains a cellular infection by a combination of dilution and hepatocyte death.

hepatic cancer pathophysiology

Achieving this would also allow a critical test of the hypothesis that curing a chronic infection would significantly reduce the risk of death due to cirrhosis, which seems likely, and due to liver cancer, hepatic cancer pathophysiology is difficult to predict, because liver cancer may occur in a liver that appears relatively healthy histologically.

Research Models HBV research generally reflects public health concerns. How can chronic infections be cured? Will eliminating the virus reduce the risk of liver cancer and premature death from liver disease? What is the mechanism of carcinogenesis? It is speculated that immune-mediated chronic injury, insertional mutagenesis, and viral proteins all may play a role.

These questions have been investigated using clinical samples and a number of model systems. Woodchucks are naturally infected with woodchuck hepatitis virus WHV Summers et al. HBV transgenic mice have been powerful tools for studying certain aspects of the antiviral immune response Guidotti and Chisari,even though these mice do hepatic cancer pathophysiology support a complete HBV infection cycle Tang and McLachlan, On occasion, chimpanzees, which are susceptible to HBV, have been used to address research issues Guidotti et al.

Among the model systems, the duck has been heavily used to understand the virus life cycle at the molecular level, to study the biology of infection, and to characterize antiviral therapies, primarily with nucleoside analogs. The wood-chuck model has been less used to study molecular biology issues, but has been employed extensively in the development of antiviral therapies and in characterization of the que es los oxiuros between chronic infection and liver cancer.

An unresolved issue arose in the latter studies. It was found that liver cancer in woodchucks is almost always associated with transcriptional activation of N-myc2 expression in the liver by insertion of viral enhancer sequences Fourel et al. Contrary to expectation, insertional activation of N-myc2 does not appear to be a correlate of liver cancer in HBV carriers. Indeed, with a few rare exceptions, it remains unclear if the frequent sporadic integration of viral DNA that characterizes an infection has a role in most liver cancers that occur in individuals chronically infected with HBV Dejean et al.

The HBV transgenic mouse, in contrast to the natural infection models, has been most heavily used to demonstrate the effects of immune cytokines, such as interferons alpha and gamma, on viral replication intermediates.

These observations seem likely to provide part of the explanation for how virus replication is shut down during the clearance of transient HBV infections. Though the relationship to natural infections is still unclear, a number of studies have shown that mice carrying the HBV transcriptional activator, X, as a transgene, are at increased risk of developing liver cancer Kim et al. These data suggest that X is in fact a viral oncogene, oxiurus nome cientifico clinical evidence to support this conclusion is still lacking, and it is difficult to address this issue in the woodchuck model, because X is needed to establish a productive infection Chen et al.

In addition to characterizing infections and therapies, the animal models have also provided, along with clinical studies, a better understanding of the difficulties of treating chronic infections with nucleoside analogs. From such studies, it has been determined that cccDNA can persist in the liver for months, and probably years, even when virus DNA synthesis is effectively inhibited Colonno et al.

Persistence of cccDNA may be attributable to two factors: 1 an inherent stability within non-dividing hepatocytes, and 2 the relatively low turnover perhaps a few percent per day of hepatocytes in most carriers. Studies with animal models have also established that the mutation rate of the viruses is quite high, with a single-base mutation prevalence of about Pult et al. Thus, drug-resistant variants, especially those requiring only one or two base changes, hepatic cancer pathophysiology likely to be present at the start of therapy.

The primary factors needed for subsequent emergence of drug-resistant variants are the hepatic cancer pathophysiology hepatic cancer pathophysiology for the hepatocyte population to become susceptible to spread of virus e. In practice, emergence of hepatic cancer pathophysiology can take from months to several years, the variation probably reflecting additional factors, including the effect of nucleoside therapy on the antiviral immune response of the host Boni et al.

Outlook Discovery of an effective HBV vaccine in the s Blumberg, led to the hope that HBV would be eliminated, or at least substantially reduced in the human population within the then foreseeable future. This still remains mostly a hope.

Two objectives still need to be fulfilled, universal vaccination Kane,and development of an effective therapy for chronic infection. Even though not everyone will be protected using the current vaccine, most would be, and the carrier incidence should decline substantially, first among the young. The goal of complete elimination seems unlikely without major advances in the treatment and elimination of chronic infections, particularly treatments that are rapid acting and cost-effective.

Australia antigen and the biology of hepatitis B. Lamivudine treatment can overcome cytotoxic T-cell hyporesponsiveness in chronic hepatitis B: new perspectives for immune therapy. The woodchuck hepatitis virus X gene is important for establishment of virus infection in woodchucks. Journal of Virology. Long-term entecavir treatment results in sustained antiviral efficacy and prolonged life span in the woodchuck hepatic cancer pathophysiology of chronic hepatitis infection.

The Journal of Infectious Diseases. Hepatitis B virus DNA integration in a sequence homologous to v-erb-A and steroid receptor genes in a hepatocellular carcinoma. Cross-resistance testing of antihepadnaviral compounds using novel recombinant baculoviruses which encode drug-resistant strains of hepatitis B virus.

Antimicrobial Agents and Chemotherapy. Entecavir therapy combined with DNA vaccination for persistent duck hepatitis B virus infection. Evidence for long-range oncogene activation by hepadnavirus insertion. Noncytolytic control of viral infections by the innate and adaptive immune response. Annual Review of Immunology.

Viral clearance without destruction of infected cells during acute HBV infection. Apoptosis and regeneration of hepatocytes during recovery from transient hepadnavirus infections. Prevalence of vaccine-induced escape mutants of hepatitis B virus in the adult population in China: a prospective study in restaurant employees.

Journal of Gastroenterology and Hepatology. New therapies for chronic hepatitis B. Journal of Viral Hepatitis. Rapid resolution of duck hepatitis B virus infections occurs after massive hepatocellular involvement.

Distribuie pe: DESCRIERE Molecular Pathology of Liver Diseases integrates the traditional knowledge of physiological and pathological processes in the liver with a balanced emphasis on fundamental concepts; timely advances in cellular and molecular mechanisms; and applied pathology. The textbook is organized into several sections, each of which includes an array of chapters that progressively and cohesively elaborate on pertinent liver biology and pathology. The first three sections hepatic cancer pathophysiology the cellular composition of the liver along with their specialized functions, and further dissect the molecular basis of the cellular processes that are so unique to the liver.

Woodchuck hepatitis virus infections: very rapid recovery hepatic cancer pathophysiology a prolonged viremia and infection of virtually every hepatocyte. Global control of primary hepatocellular carcinoma with hepatitis B vaccine: The contributions of research in Taiwan.

HBx gene of hepatitis B virus induces liver cancer in transgenic mice. Long-term ganciclovir chemotherapy for congenital duck hepatitis B virus infection in vivo: Effect on intrahepatic-viral DNA, RNA, and protein expression. Hepatitis B virus X protein acts as a tumor promoter in development of diethylnitrosamine-induced preneoplastic lesions.

Molecular Genetics of Liver Neoplasia - asspub.ro

Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. Lack of effect of antiviral therapy in nondividing hepatocyte cultures on the closed circular DNA of woodchuck hepatitis virus. How will we use the new antiviral agents for hepatitis B? Current Gastroenterology Reports.

Încărcat de

Hepatocarcinogenicity of the woodchuck hepatitis virus. Proceedings of the National Academy of Sciences. Frequency of spontaneous mutations in an avian hepadnavirus infection. Hepatitis B virus biology. Microbiology and Molecular Biology Reviews.

Hepatitis B Virus Replication

Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediate. A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Avian and mammalian hepadnaviruses have distinct transcription factor requirements for viral replication. Adefovir nephrotoxicity: possible role of mitochondrial DNA depletion.

helmintii la copii human papillomavirus results in

Human Hpv tunete ferfiaknal. The hepatitis B virus X gene potentiates c-myc-induced liver oncogenesis in transgenic mice.

Formation of the pool of covalently closed circular viral DNA in hepadnavirus-infected cells.

Molecular Pathology of Liver Diseases

Hepadnavirus P protein utilizes a tyrosine residue in the TP domain to prime reverse transcription. Hepadnavirus integration: mechanisms of activation of the N-myc2 retrotransposon in woodchuck liver tumors. Predictions of the emergence of vaccine-resistant hepatitis B in The Gambia using a mathematical model.

Epidemiology and Infection.

Low dynamic state of a viral competition in a chronic avian hepadnavirus infection. A virus possibly associated with hepatitis and hepatoma in ducks. Shanghai Medical Journal.

hepatic cancer pathophysiology verme oxiurus imagens

Kinetics of hepadnavirus loss from the liver during inhibition of viral DNA synthesis. Reverse transcription in hepatitis B viruses is primed by a tyrosine residue of the polymerase.

Woodchuck hepatitis virus X protein is required for viral infection in vivo.