Non human papillomavirus,
- HUMAN PAPILLOMA VIRUS – CE METODA DE TESTARE FOLOSIM?
- synlab: Cancerul de col uterin
- Avantajele acestui test extrem de laborios sint foarte clare:
- Facing Human Papillomavirus (HPV) – Texas Children's Hospital
- Metoda cea mai sensibila (pina la 100%!!!), mai specifica si mai cuprinzatoare este:
- RRML - α-HPV positivity analysis in a group of patients with melanoma and non-melanoma skin cancers
In addition to tobacco and alcohol abuse, certain viruses have been associated with squamous cell carcinoma SCC of the head and neck, causing alterations in DNA.
HUMAN PAPILLOMA VIRUS – CE METODA DE TESTARE FOLOSIM?
It has been demonstrated that the human papillomavirus HPV type 16, a subtype of the human papillomavirus, is present in the oropharyngeal carcinomas of non-smokers patients inclusive. HPV-infected cells express some viral proteins encoded by genes called E6 and E7, and can inactivate p53 protein and the retinoblastoma-type protein RBP involved in the regulation of proliferation and cell death.
Materials and method. We present an immunohistochemical study conducted to identify non human papillomavirus tumour markers in tonsillar SCC.
We present the statistically significant correlations between the presence of immunohistochemical markers and studied local recurrence, lymph node recurrence and risk of a second cancer in the aerodigestive upper tract. The demonstration of HPV in tonsillar tumour tissue requires in situ hybridization or polymerase chain reaction PCR for the evidence of viral genome included into the host cell. The practical implications of an etiologic role of HPV in head and neck cancer generally and in tonsillar SCC in particular remains in question and is in relate with prognosis, treatment and prevention.
În afară de consumul de tutun şi abuzul de alcool, anumite virusuri au fost non human papillomavirus cu carcinomul cu celule scuamoase CCS al capului şi gâtului, cauzând alterări la nivelul ADN-ului. Este dovedit că virusul papiloma uman HPVtipul 16, este prezent la nivelul carcinoamelor orofaringiene inclusiv în cazul nefumătorilor.
Celulele non human papillomavirus cu HPV exprimă unele proteine virale codate de genele denumite E6 şi E7 şi pot inactiva proteina p53 şi proteina de tip retinoblastom RBP implicate în reglarea proliferării şi morţii celulare. Materiale şi metodă. Prezentăm un studiu imunohistochimic realizat cu scopul de a identifica markeri tumorali semnificativi în CCS de amigdală.
Prezentăm corelaţiile semnificative statistic între prezenţa markerilor imunohistochimici şi recurenţa locală, recurenţa nodulilor limfatici şi riscul apariţiei unui al doilea cancer în tractul aerodigestiv superior. Punerea în evidenţă a HPV-ului în ţesutul tumoral amigdalian necesită hibridizare in situ şi reacţie de polimerizare în lanţ PCR pentru punerea în evidenţă a genomului viral conţinut în celula-gazdă.
Implicaţiile practice ale unui rol etiologic al HPV-ului în cancerele de cap şi gât, în general, şi în CCS de amigdală, în particular, reprezintă un subiect în dezbatere, fiind în relaţie cu prognosticul, tratamentul şi prevenţia acestor tipuri de cancere. Cuvinte cheie carcinomul cu celule scuamoase de amigdală CCS HPV markeri tumorali Introduction The tonsillar squamous cell carcinoma SCC is non human papillomavirus a public health problem because of its rising incidence in the last 20 years, in contrast to the decreasing incidence of carcinomas in other subsites of head and neck associated to the reduced prevalence of smoking.
Если небо было недосягаемо, а путь по земле - перекрыт, что оставалось.
Он ничего не добавил к сказанному, но оба поняли друг - Меня это также заботило, - признался Хилвар, - но я надеюсь, что когда наши народы вновь узнают друг друга, эта проблема постепенно разрешится сама .
These tumours of oral cavity, oropharynx, larynx, hypopharynx and sinonasal region are linked by common characteristics, including a male predominant appearance in the 5th-6th decade of life, an important etiological link non human papillomavirus tobacco, alcohol use or betel non human papillomavirus chewing, and a histopathological resemblance 1.
Data regarding the epidemiology revealed that in Romania the oropharyngeal cancer represents 2. Non human papillomavirus France, during the last 30 years, the mortality in oral and oropharyngeal cancer increased by three times 1. As in cervical cancers, the oropharyngeal infection with HPV is a sexually transmitted disease which involves some particularities of sexual behaviour: a large number of vaginal non human papillomavirus partners, cancer plamani doare and anal sex.
synlab: Cancerul de col uterin
The recent increasing of OPSCC incidence may reflect the social changes regarding sexual behaviour in the modern world 6. The anatomical sites preferred by HPV in oropharynx are the tonsils and the non human papillomavirus, because of the unique presence of transitional mucosa in oropharynx and particular in tonsillar tissue, which presents important histological similarities with the cervical mucosa.
Tonsillar epithelium invagination may favour virus capture and promote its access to basal cells the non human papillomavirus dividing cells in the epithelium. The tonsillar tissue could be a reservoir for HPV in the upper aero digestive tract.
Avantajele acestui test extrem de laborios sint foarte clare:
We had two premises for our study on tonsillar cancers. The second consists in the fact that mutagens such as tobacco, alcohol and HPV viral oncogenes E6 and E7 induce dysfunctions of two major mechanisms of cellular cycle, which involves the p53 and RBP tumoral suppressor genes 2.
Metodele de testare pentru HPV cunoscute pina in prezent prezinta dezavantaje care nu trebuie neglijate: detecteaza un numar relativ mic de tipuri de HPV comparativ cu cele existente 37 de tipuri, fata de non human papillomavirus peste de tipuri cunoscutese aplica doar pentru prelevate cervicale in mediu lichid excluzind astfel leziunile anale, oro-faringiene, conjunctivale, epidermice, laringealeau sensibilitate limitata pentru unele tipuri, limita de detectie ajunge si non human papillomavirus de copii ADN, ceea ce sugereaza un numar relativ mare de cazuri fals negative, datorate fie recoltarii unui numar mic de celule, fie infectarii cu virus a unui numar mic de celule. Laboratoarele synlab utilizeaza acum o metoda de testare a HPV care exclude toate aceste inconveniente.
Materials and method We made an immunohistochemical retrospective study between andaiming to identify any correlations between tumoral markers and the evolution and prognosis in tonsillar SCC.
Materials We studied 52 cases of patients diagnosed with tonsillar SCC.
Facing Human Papillomavirus (HPV) – Texas Children's Hospital
We had a first group Group I with 25 cases, where the positive diagnose was made by biopsy and these patients had radiotherapy as first curative method of treatment.
We had a second group Group II with 27 cases, where the positive diagnose was made on surgical specimens and these patients had surgery as the first curative method of treatment.
- Cancer de vezica urinara recidiva
Все, что он знал и любил, осталось в Диаспаре; возможно, он никогда больше не увидит свой мир, даже если впереди никакие опасности не грозят.
С уходом солнца лужи черной тени, плескавшиеся меж дюн, сразу же стремительно слились в одно необозримое озеро тьмы.
The two groups were similar regarding age and gender distribution. The dilutions and markers specifications are revealed in Table 1.
Metoda cea mai sensibila (pina la 100%!!!), mai specifica si mai cuprinzatoare este:
We also studied lymphocyte populations CD4, CD8, and populations of dendritic cells in tumour tissue. Table 1. The dilutions and markers specifications For the immunohistochemical identification of tumoral antigens we used the three-stadial indirect method Avidine-Biotine-Peroxidase ABPafter Hsu and colab.
Results The gender repartition of cases was: 47 male cases non human papillomavirus 5 female cases. The age repartition of cases was: two cases between years old, 14 cases between years old, 21 cases between years old, 10 cases between years old, and five cases between years old.
RRML - α-HPV positivity analysis in a group of patients with melanoma and non-melanoma skin cancers
The correlation coefficient between the two sets of data, corresponding to Group I and Group II, was 0. In both groups, we had 48 smoker patients, representing The patients who were both smokers and alcohol consumers represented We studied the tumoral markers on 52 cases of squamous cell carcinoma.
Thirty-eight cases were well differentiated carcinoma and 14 cases were medium differentiated carcinoma. We present the results, that we considered immunohistochemically valid and statistically significant Table 2.
Table 2. The distribution of tumoral markers in specimens of SCC studied We realised a correlation between the presence of the tumoral marker of a certain type positive and slowly positive results and the post-therapeutic evolution — local recurrence, nodal relapse, the occurrence of second cancers in upper aerodigestive upper ways and distance metastases.
We have had patients who had more than one recurrence in the same time. Our purpose was to non human papillomavirus the correlations between markers of evolution and prognosis in tonsillar SCC. Our results indicate p53 protein and RBP protein as tumoral markers of unfavourable prognosis for post-therapeutic evolution in tonsillar SCC.
For TGFa, we can make a correlation between its level in tumoral tissue and the risk of loco-regional relapse. For the HPV identification in tumoral tissue, we used the identification of capsid p16 protein, so we cannot make definitive conclusions referring at the presence or absence of HPV in the tumoral tissue for patients with tonsillar Non human papillomavirus.
But non human papillomavirus realised a correlation between the presence of HPV and the type of post-therapeutic evolution Figures Figure 1. The presence of RBP protein 48 positive and slowly positive cases was associated with local recurrence in 29 cases The presence of TGF protein 41 positive and slowly positive cases was associated with local recurrence in 18 cases The presence of HPV capsid protein 14 positive cases was associated with local non human papillomavirus in nine cases Figure 6.
Tumoral markers in evolution of tonsillar SCC result of our retrospective study From our data, we can certify as prognostic factors in tonsillar SCC: T stage, N stage, performing or not an elective type of clinical negative neck N0, type of neck dissection, the total dose of radiotherapy.
We cannot make non human papillomavirus significant conclusions referring to the HPV presence in tumoral tissue in tonsillar SCC and long-term prognosis.